| 质粒名称: | pMSCV-hygro-STING R232 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 102608 |
| 存储实验室: | Nicolas Manel |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCV-hygro |
| 完整质粒大小: | 8085bp |
| 载体类型: | 哺乳细胞表达, Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | STING |
| 物种来源: | H. sapiens (human) |
| 基因突变: | R232 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | unknown (unknown if destroyed) |
| 5’测序引物 | MSCV |
|
文献引用方法 |
|
| 材料和方法部分: | pMSCV-hygro-STING R232 was a gift from Nicolas Manel (Addgene plasmid # 102608 ; http://n2t.net/addgene:102608 ; RRID:Addgene_102608) |
| 参考文献部分: | Transmission of innate immune signaling by packaging of cGAMP in viral particles. Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N. Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30. 10.1126/science.aab3628 PubMed 26229115 |
买家导航
pMSCV-hygro-STING R232载体质粒基本信息
pMSCV-hygro-STING R232质粒图谱载体图谱和pMSCV-hygro-STING R232载体序列质粒序列多克隆位点信息
pMSCV-hygro-STING R232质粒载体简介
pMSCV-hygro-STING R232质粒序列载体序列
LOCUS pMSCV-hygro-STING R232 8085 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Retroviral vector to expression STING R232.
ACCESSION .
VERSION .
KEYWORDS pMSCV-hygro-STING R232
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8085)
AUTHORS Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M,
Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N
TITLE Transmission of innate immune signaling by packaging of cGAMP in
viral particles.
JOURNAL Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628.
Epub 2015 Jul 30.
PUBMED 26229115
REFERENCE 2 (bases 1 to 8085)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8085
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 94..610
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 674..1015
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 1082..1498
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1428..1450
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1466..1482
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
promoter 2673..3172
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2708..2731)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3105..3124
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3190..4215
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
LTR 4573..5087
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 5249..5265
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5273..5303)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5318..5339
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5456..5473)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5627..6215)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5707..5726)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6386..7246)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7009..7028
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7247..7351)
/gene="bla"
/label=AmpR promoter
primer_bind 7419..7437
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7475..7497)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7597..7616
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7810..7832
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7960..7979
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
61 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
121 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
181 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
241 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
301 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
361 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
421 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
481 tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa
541 tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg
601 gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc
661 cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt
721 ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat
781 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
841 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
901 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
961 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
1021 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
1081 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
1141 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
1201 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
1261 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
1321 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
1381 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
1441 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
1501 aattagatcc atgccccact ccagcctgca tccatccatc ccgtgtccca ggggtcacgg
1561 ggcccagaag gcagccttgg ttctgctgag tgcctgcctg gtgacccttt gggggctagg
1621 agagccacca gagcacactc tccggtacct ggtgctccac ctagcctccc tgcagctggg
1681 actgctgtta aacggggtct gcagcctggc tgaggagctg cgccacatcc actccaggta
1741 ccggggcagc tactggagga ctgtgcgggc ctgcctgggc tgccccctcc gccgtggggc
1801 cctgttgctg ctgtccatct atttctacta ctccctccca aatgcggtcg gcccgccctt
1861 cacttggatg cttgccctcc tgggcctctc gcaggcactg aacatcctcc tgggcctcaa
1921 gggcctggcc ccagctgaga tctctgcagt gtgtgaaaaa gggaatttca acgtggccca
1981 tgggctggca tggtcatatt acatcggata tctgcggctg atcctgccag agctccaggc
2041 ccggattcga acttacaatc agcattacaa caacctgcta cggggtgcag tgagccagcg
2101 gctgtatatt ctcctcccat tggactgtgg ggtgcctgat aacctgagta tggctgaccc
2161 caacattcgc ttcctggata aactgcccca gcagaccggt gaccgtgctg gcatcaagga
2221 tcgggtttac agcaacagca tctatgagct tctggagaac gggcagcggg cgggcacctg
2281 tgtcctggag tacgccaccc ccttgcagac tttgtttgcc atgtcacaat acagtcaagc
2341 tggctttagc cgggaggata ggcttgagca ggccaaactc ttctgccgga cacttgagga
2401 catcctggca gatgcccctg agtctcagaa caactgccgc ctcattgcct accaggaacc
2461 tgcagatgac agcagcttct cgctgtccca ggaggttctc cggcacctgc ggcaggagga
2521 aaaggaagag gttactgtgg gcagcttgaa gacctcagcg gtgcccagta cctccacgat
2581 gtcccaagag cctgagctcc tcatcagtgg aatggaaaag cccctccctc tccgcacgga
2641 tttctcttga ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca
2701 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
2761 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
2821 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
2881 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
2941 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
3001 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
3061 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
3121 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat
3181 cccgccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
3241 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
3301 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
3361 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
3421 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
3481 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
3541 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
3601 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
3661 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
3721 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
3781 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
3841 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
3901 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
3961 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
4021 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
4081 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
4141 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
4201 agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc
4261 cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca
4321 cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag
4381 tgattcaggc ccttctggat tgtgttggtc cccagggcac gattgtcatg cccacgcact
4441 cgggtgatct gactgatccc gcagattgga gatcgccgcc cgtgcctgcc gattgggtgc
4501 agatccgtcg acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag
4561 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
4621 ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa
4681 cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
4741 ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa
4801 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
4861 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
4921 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc
4981 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
5041 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc
5101 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa
5161 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc
5221 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac
5281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
5341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
5401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
5461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
5521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
5581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
5641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
5701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
5761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
5821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
5881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
5941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6061 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6181 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6241 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
6301 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
6361 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
6421 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
6481 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
6541 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
6601 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
6661 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
6721 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
6781 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
6841 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
6901 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
6961 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
7021 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
7081 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
7141 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
7201 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
7261 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
7321 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
7381 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
7441 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
7501 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
7561 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
7621 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
7681 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
7741 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
7801 cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga
7861 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
7921 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
7981 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggcg
8041 attagtccaa tttgttaaag acaggatatc agtggtccag gctct
//
